Multiplex Assay:Article Title: Nonrandom Selection and Multiple Blood Feeding of Human Hosts by Anopheles Vectors: Implications for Malaria Transmission in Papua New Guinea
Article Snippet: .. The assay used a multiplex PCR kit (PowerPlex Fusion System; catalog number, DC2402; Promega, Madison, WI) that contained fluorophore-labeled primer pairs that co-amplified 22 autosomal microsatellite loci (D3S1358, D1S1656, D2S441, D10S1248, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, vWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433, FGA, D22S1045), one Y chromosome-linked microsatellite locus (DYS391), and one locus serving as a sex-specific marker (Amel). ..
Article Title: Nonrandom Selection and Multiple Blood Feeding of Human Hosts by Anopheles Vectors: Implications for Malaria Transmission in Papua New Guinea.
Article Snippet: .. The assay used a multiplex PCR kit (PowerPlex Fusion System; catalog number, DC2402; Promega, Madison, WI) that contained fluorophore-labeled primer pairs that co-amplified 22 autosomal microsatellite loci (D3S1358, D1S1656, D2S441, D10S1248, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, vWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433, FGA, D22S1045), one Y chromosome-linked microsatellite locus (DYS391), and one locus serving as a sex-specific marker (Amel).60 The primers that target these human loci do not amplify other vertebrate species commonly used by Anopheles mosquitoes as blood hosts except three nonhuman primates (chimpanzee, gorilla, and macaque), which are not found in PNG, although their genetic profiles are easily distinguished from those of humans.60 The PCR reaction mixture (10mL reaction volume) consisted of 6mL of water, 1mL of 5x Fusion Master Mix, 1mL of 5x Fusion Primer Mix, 0.5mL of 10x PCR Buffer (catalog number, N8080010; Thermo Fisher Scientific), 0.5mL of 25 mM MgCl2 (catalog number, N8080010; Thermo Fisher Scientific), and 1mL of DNA (for samples with low DNA concentration, 4mL were used with the water volume adjusted to 3mL). ..
Polymerase Chain Reaction:Article Title: Nonrandom Selection and Multiple Blood Feeding of Human Hosts by Anopheles Vectors: Implications for Malaria Transmission in Papua New Guinea
Article Snippet: .. The assay used a multiplex PCR kit (PowerPlex Fusion System; catalog number, DC2402; Promega, Madison, WI) that contained fluorophore-labeled primer pairs that co-amplified 22 autosomal microsatellite loci (D3S1358, D1S1656, D2S441, D10S1248, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, vWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433, FGA, D22S1045), one Y chromosome-linked microsatellite locus (DYS391), and one locus serving as a sex-specific marker (Amel). ..
Article Title: Nonrandom Selection and Multiple Blood Feeding of Human Hosts by Anopheles Vectors: Implications for Malaria Transmission in Papua New Guinea.
Article Snippet: .. The assay used a multiplex PCR kit (PowerPlex Fusion System; catalog number, DC2402; Promega, Madison, WI) that contained fluorophore-labeled primer pairs that co-amplified 22 autosomal microsatellite loci (D3S1358, D1S1656, D2S441, D10S1248, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, vWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433, FGA, D22S1045), one Y chromosome-linked microsatellite locus (DYS391), and one locus serving as a sex-specific marker (Amel).60 The primers that target these human loci do not amplify other vertebrate species commonly used by Anopheles mosquitoes as blood hosts except three nonhuman primates (chimpanzee, gorilla, and macaque), which are not found in PNG, although their genetic profiles are easily distinguished from those of humans.60 The PCR reaction mixture (10mL reaction volume) consisted of 6mL of water, 1mL of 5x Fusion Master Mix, 1mL of 5x Fusion Primer Mix, 0.5mL of 10x PCR Buffer (catalog number, N8080010; Thermo Fisher Scientific), 0.5mL of 25 mM MgCl2 (catalog number, N8080010; Thermo Fisher Scientific), and 1mL of DNA (for samples with low DNA concentration, 4mL were used with the water volume adjusted to 3mL). ..
Marker:Article Title: Nonrandom Selection and Multiple Blood Feeding of Human Hosts by Anopheles Vectors: Implications for Malaria Transmission in Papua New Guinea
Article Snippet: .. The assay used a multiplex PCR kit (PowerPlex Fusion System; catalog number, DC2402; Promega, Madison, WI) that contained fluorophore-labeled primer pairs that co-amplified 22 autosomal microsatellite loci (D3S1358, D1S1656, D2S441, D10S1248, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, vWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433, FGA, D22S1045), one Y chromosome-linked microsatellite locus (DYS391), and one locus serving as a sex-specific marker (Amel). ..
Article Title: Nonrandom Selection and Multiple Blood Feeding of Human Hosts by Anopheles Vectors: Implications for Malaria Transmission in Papua New Guinea.
Article Snippet: .. The assay used a multiplex PCR kit (PowerPlex Fusion System; catalog number, DC2402; Promega, Madison, WI) that contained fluorophore-labeled primer pairs that co-amplified 22 autosomal microsatellite loci (D3S1358, D1S1656, D2S441, D10S1248, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, vWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433, FGA, D22S1045), one Y chromosome-linked microsatellite locus (DYS391), and one locus serving as a sex-specific marker (Amel).60 The primers that target these human loci do not amplify other vertebrate species commonly used by Anopheles mosquitoes as blood hosts except three nonhuman primates (chimpanzee, gorilla, and macaque), which are not found in PNG, although their genetic profiles are easily distinguished from those of humans.60 The PCR reaction mixture (10mL reaction volume) consisted of 6mL of water, 1mL of 5x Fusion Master Mix, 1mL of 5x Fusion Primer Mix, 0.5mL of 10x PCR Buffer (catalog number, N8080010; Thermo Fisher Scientific), 0.5mL of 25 mM MgCl2 (catalog number, N8080010; Thermo Fisher Scientific), and 1mL of DNA (for samples with low DNA concentration, 4mL were used with the water volume adjusted to 3mL). ..
Concentration Assay:Article Title: Nonrandom Selection and Multiple Blood Feeding of Human Hosts by Anopheles Vectors: Implications for Malaria Transmission in Papua New Guinea.
Article Snippet: .. The assay used a multiplex PCR kit (PowerPlex Fusion System; catalog number, DC2402; Promega, Madison, WI) that contained fluorophore-labeled primer pairs that co-amplified 22 autosomal microsatellite loci (D3S1358, D1S1656, D2S441, D10S1248, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, vWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433, FGA, D22S1045), one Y chromosome-linked microsatellite locus (DYS391), and one locus serving as a sex-specific marker (Amel).60 The primers that target these human loci do not amplify other vertebrate species commonly used by Anopheles mosquitoes as blood hosts except three nonhuman primates (chimpanzee, gorilla, and macaque), which are not found in PNG, although their genetic profiles are easily distinguished from those of humans.60 The PCR reaction mixture (10mL reaction volume) consisted of 6mL of water, 1mL of 5x Fusion Master Mix, 1mL of 5x Fusion Primer Mix, 0.5mL of 10x PCR Buffer (catalog number, N8080010; Thermo Fisher Scientific), 0.5mL of 25 mM MgCl2 (catalog number, N8080010; Thermo Fisher Scientific), and 1mL of DNA (for samples with low DNA concentration, 4mL were used with the water volume adjusted to 3mL). ..
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